RNA Competition in RNA-DNA Hybridization Systems
نویسندگان
چکیده
منابع مشابه
Patterns of RNA synthesis in T5-infected cells. I. As studied by the technique of DNA-RNA hybridization-competition.
The RNA-labeling patterns obtained after T5 infection of Escherichia coli F agree with the patterns of protein labeling published by McCorquodale and Buchanan.(1) Three distinct classes of RNA formed sequentially during the period of viral development can be recognized by the DNA-RNA hybridization-competition technique. Class I RNA is formed within 5 minutes after the beginning of viral metabol...
متن کاملHybridization of chromosomal RNA to native DNA.
Chromosomal RNA, which is associated with chromosomal proteins in the chromosomes of higher organisms, possess the ability to hybridize to homologous native DNA. The proportion of native DNA thus hybridized is similar to the proportion of denatured DNA which hybridizes with chromosomal RNA, and both are similar to the proportions of chromosomal RNA and DNA in native chromatin.
متن کاملEnhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase
Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...
متن کاملIndication of Gene Amplification in Rhynchosciara by Rna-dna Hybridization
nism of cell differentiation was recently demonstrated in the case of ribosomal RNA cistrons of amphibian oocytes (1-3), and it seems that this phenomenon is of widespread occurrence (4) . In somatic cells the evidence for amplification thus far is based on cytological observations . The best known cases are those of Rhynchosciara and Sciara ; in the prepupal phase of these insects, it was poss...
متن کاملFluorescent in situ hybridization of mitochondrial DNA and RNA.
To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science
سال: 1968
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.162.3854.699